SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Maróstica,P.J.C.; Raskin,S.; Abreu-e-Silva,F.A..

Sixty-one cystic fibrosis patients admitted for check-up or antibiotic treatment were enrolled for genetic and clinical evaluation. Genetic analysis was performed on blood samples stored on neonatal screening cards using PCR techniques to determine the presence of DF508 mutations. Clinical evaluation included Shwachman and Chrispin-Norman scores, age at onset of symptoms and diagnosis, spirometry, awake and sleep pulse oximetry, hyponychial angle measurement and presence of chronic Pseudomonas aeruginosa colonization. Eighteen patients (29.5%) were homozygous for the DF508 mutation, 26 (42.6%) had one DF508 mutation and 17 (27.9%) were...

Antioxidant activity of the microalga Spirulina maxima

Palavras-chave: Cystic fibrosis <; FONT FACE=Symbol>; D<; /font>; F508 gene mutation genotype phenotype.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400009

Miranda,M.S.; Cintra,R.G.; Barros,S.B.M.; Mancini-Filho,J..

Spirulina maxima, which is used as a food additive, is a microalga rich in protein and other essential nutrients. Spirulina contains phenolic acids, tocopherols and ß-carotene which are known to exhibit antioxidant properties. The aim of the present study was to evaluate the antioxidant capacity of a Spirulina extract. The antioxidant activity of a methanolic extract of Spirulina was determined in vitro and in vivo. The in vitro antioxidant capacity was tested on a brain homogenate incubated with and without the extract at 37oC. The IC50 (concentration which causes a 50% reduction of oxidation) of the extract in this system was 0.18 mg/ml. The in vivo antioxidant capacity was evaluated in plasma and liver of animals receiving a daily dose of 5 mg for 2 and...

Bacteria isolated from a sugarcane agroecosystem: their potential production of polyhydroxyalcanoates and resistance to antibiotics

Palavras-chave: Microalgae antioxidant activity Spirulina maxima phenolic compounds ß-carotene <; FONT FACE=Symbol>; A<; /FONT>; -tocopherol.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800007

Rev. Microbiol.

Lima,Teresa Cristina S. de; Grisi,Breno M.; Bonato,M. Christina M..

In this investigation, a sugarcane agroecosystem at a coastal tableland, in the northeast of Brazil, was screened to obtain bacteria strains able to synthesize poly-b-hydroxyalkanoates (PHA), using sucrose as the main carbon source. The potential to synthesize PHA was tested qualitatively by Sudan Black staining of colonies growing in different carbon sources: sucrose, glucose, fructose, propionate and cellulose. In a typical sugarcane crop management system, the plantation is burned before harvesting and vinasse, a byproduct of alcohol production, is used in a fertirrigation system causing, probably, selective pressures on the microbiota of natural environments. Eightytwo bacteria strains, belonging to 16 different...

Carbono e nitrogênio em solo de uma cronossequência de floresta tropical - pastagem de Paragominas

Palavras-chave: Soil bacteria poly-<; FONT FACE=Symbol>; B<; /font>; -hydroxyalkanoate PHA resistance to antibiotics sugarcane agroecosystem vinasse.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300006

Scientia Agricola

Bernoux,Martial; Feigl,Brigitte Josefine; Cerri,Carlos Clemente; Geraldes,Ana Paula de Arauda; Fernandes,Silvana Aparecida Pavan.

Amostras de terra de áreas desmatadas há 4, 10 e 15 anos e utilizadas como pastagem e uma floresta nativa na região de Paragominas-PA, foram analisadas quanto à constituição isotópica e teor de carbono (C) e nitrogênio (N) com o objetivo de quantificar os estoques de C e N e as alterações devidas a mudanças do uso da terra. O conteúdo de C e N aumentaram ao longo do tempo de uso do solo como pastagem. Após 4 e 15 anos de cultivo o conteúdo de C aumentou 0,28 e 0,86 kg.m-2 na camada superficial 0-30 cm do solo; a pastagem de 10 anos ficou estável (diminuição de 0,02 kg.m-2) em relação à mata natural para a camada 0-30 cm, mas diminuiu de 0,15 na camada 0-10 cm. Na camada superficial 0-30 cm, a matéria orgânica da área sob mata natural é composta por 2,6...

Composição química de sementes de trigo (Triticum aestivum L.) submetidas a estresse salino na germinação

Palavras-chave: Matéria orgânica pastagem floresta Amazônica <; FONT FACE=Symbol>; D<; /font>; 13C carbono nitrogênio.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000400003

Rev. bras. sementes

Silva,Rodrigo Nascimento da; Duarte,Gisela Loureiro; Lopes,Nei Fernandes; Moraes,Dario Munt de; Pereira,Ana Lúcia de Almeida.

O objetivo do experimento foi determinar algumas alterações bioquímicas de sementes de duas cultivares de trigo (BRS 177 e BRS 179) sob influência de diferentes níveis de sal (zero, 15, 30, 45 e 60mM NaCl) no processo de germinação. O ensaio foi realizado para avaliar alterações na atividade da a-amilase e nos teores de amido, açúcares redutores, carboidratos solúveis totais e proteínas causadas pelo estresse salino. A atividade da a-amilase e os teores carboidratos solúveis totais, açúcares redutores e proteínas são reduzidos, porém o conteúdo de amido aumenta com o incremento da salinidade, em ambas cultivares de trigo. Em todas as concentrações de NaCl, os componentes químicos nas sementes da cultivar BRS 179 são maiores do que os da cv. BRS 177,...

Cytokine profiles during experimental Chagas' disease

Palavras-chave: Triticum aestivum L. <; FONT FACE=Symbol>; A<; /font>; -amilase amido açúcares proteínas alterações bioquímicas sal.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222008000100027

Morato,M.J.F.; Colley,D.G.; Powell,M.R..

People infected with Trypanosoma cruzi remain so for life, yet only 30-40% of these individuals develop characteristic chagasic cardiomyopathies. Similarly, when infected with the Brazilian strain of T. cruzi, DBA/2 mice develop severe cardiac damage while B10.D2 mice do not. To better understand the immunological parameters that may be involved in the disease process, we have used this murine model (DBA/2 vs B10.D2) and compared the changes in cytokine production during the course of infection with T. cruzi. Concanavalin A (Con A) stimulation of spleen cells harvested during the acute phase (day 30) resulted in similarly high levels of IFN-g in both mouse strains. However, the amount of IFN-<FONT...

Cytokines as determinants of resistance and pathology in human Schistosoma mansoni infection

Palavras-chave: Experimental Chagas' disease Trypanosoma cruzi cytokines IFN-<; FONT FACE=Symbol>; G<; /font>; IL-10 IL-4.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100016

Corrêa-Oliveira,R.; Malaquias,L.C.C.; Falcão,P.L.; Viana,I.R.C.; Bahia-Oliveira,L.M.G.; Silveira,A.M.S.; Fraga,L.A.O.; Prata,A.; Coffman,R.L.; Lambertucci,J.R.; Cunha-Melo,J.R.; Martins-Filho,O.A.; Wilson,R.A.; Gazzinelli,G..

The role of different cytokines in the peripheral blood mononuclear cell (PBMC) proliferative response and in in vitro granuloma formation was evaluated in a cross-sectional study with patients with the different clinical forms and phases of Schistosoma mansoni infection, as well as a group of individuals "naturally" resistant to infection named normal endemic (NE). The blockage of IL-4 and IL-5 using anti-IL-4 and anti-IL-5 antibodies significantly reduced the PBMC proliferative response to soluble egg (SEA) and adult worm (SWAP) antigens in acute (ACT), chronic intestinal (INT) and hepatosplenic (HS) patients. Similar results were obtained in the in vitro granuloma formation. Blockage of IL-10 had no significant effect on either assay using PBMC from ACT...

Does plasma ANP participate in natriuresis induced by a-MSH?

Palavras-chave: Schistosoma mansoni human schistosomiasis mansoni cytokines IL-10 IL-4 IFN-<; FONT FACE=Symbol>; G<; /font>.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100024

a-Melanocyte-stimulating hormone (a-MSH; 0.6 and 3 nmol) microinjected into the anteroventral region of the third ventricle (AV3V) induced a significant increase in diuresis without modifying natriuresis or kaliuresis. Intraperitoneal (ip) injection of a-MSH (3 and 9.6 nmol) induced a significant increase in urinary sodium, potassium and water excretion. Intraperitoneal (3 and 4.8 nmol) or iv (3 and 9.6 nmol) administration of a-MSH did not induce any significant changes in plasma atrial natriuretic peptide (ANP), suggesting that the natriuresis, kaliuresis and diuresis induced by the systemic action of...

Effect of the injection of an extract of Ascaris suum on macrophage activation during the early phase of Mycobacterium bovis BCG infection in C57Bl/6 mice

Palavras-chave: <; FONT FACE=Symbol>; A<; /font>; -MSH AV3V ANP natriuresis kaliuresis diuresis.

Ano: 1997

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400005

Ferreira,A.P.; Aarestrup,F.M.; Bonecini-Almeida,M.G.; Souza,E.E.; Gomes,E.A.; Corrêa,J.O.A.; Teixeira,H.C..

Injection of an Ascaris suum extract (Asc) affects both the humoral and cellular immune responses to unrelated antigens when it is co-administered with these antigens. In the present study we evaluated the effect of Asc on macrophage activation in the early phase of Mycobacterium bovis BCG (Pasteur strain TMCC 1173) infection in C57Bl/6 mice. C57Bl/6 mice were injected intraperitoneally (ip) with 0.1 mg BCG (BCG group) or BCG plus 1 mg Asc (BCG + Asc group). The peritoneal exudates were obtained at 2, 7 and 14 days after infection. The numbers of IFN-g-secreting cells were assessed by the ELISPOT assay. Nitric oxide (NO) production was measured by the Griess method and by the evaluation of NADPH diaphorase activity in...

Effect of toxin-g from Tityus serrulatus scorpion venom on gastric emptying in rats

Palavras-chave: Mycobacterium bovis Ascaris suum macrophage activation TNF-<; FONT FACE=Symbol>; A<; /FONT>; NADPH diaphorase.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100014

Bucaretchi,F.; Vinagre,A.M.; Chavéz-Olórtegui,C.; Collares,E.F..

The effect of toxin-g from Tityus serrulatus scorpion venom on the gastric emptying of liquids was studied in 176 young adult male Wistar rats (2-3 months of age) divided into subgroups of 8 animals each. Toxin-g was injected iv at doses of 25, 37.5, 50 or 100 µg/kg and the effect on gastric emptying was assessed 30 min and 8 h later. A time-course study was also performed by injecting 50 µg of toxin-g /kg and measuring the effect on gastric emptying at times 0.25, 0.5, 1, 2, 4, 8, 24 and 48 h post-venom. Each envenomed animal was paired with its saline control and all received a saline test meal solution containing phenol red (60 µg/ml) as...

Eosinophil-active chemokines: assessment of in vivo activity

Palavras-chave: Gastric emptying Tityus serrulatus scorpion venom toxin-<; FONT FACE=Symbol>; G<; /font>.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400009

Teixeira,M.M..

The selective recruitment of eosinophils in tissue is a striking feature of allergic diseases. Recently, a family of chemoattractant molecules, namely chemokines, has been described which potently activates eosinophil function in vitro. We have developed a murine model of eosinophil recruitment to compare the relative potency and efficacy of chemokines in vivo. Of the chemokines tested, only eotaxin and MIP-1a induced significant accumulation of eosinophils in vivo, but eotaxin was more effective than MIP-1a. Chemokines, especially eotaxin acting via the CCR-3 receptor, may have a fundamental role in determining selective eosinophil recruitment in vivo

Estimação de parâmetros genéticos em bovinos de corte utilizando os métodos de máxima verossimilhança restrita e Â

Palavras-chave: Eosinophils chemokines eotaxin MIP-1<; FONT FACE=Symbol>; A<; /font>; Chemokine receptors.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100003

R. Bras. Zootec.

Melis,Marcelo Hessel Van; Eler,Joanir Pereira; Silva,Josineudson Augusto II de Vasconcelos; Ferraz,José Bento Sterman.

Com o objetivo de comparar as estimativas de parâmetros genéticos obtidas pelos métodos de máxima verossimilhança restrita (REML) e Â, foram realizadas análises uni-características sob modelo animal, para os quais foi utilizado um conjunto de dados da raça Nelore. Os modelos estatísticos incluíram os efeitos fixos de grupo de contemporâneos (fazenda, ano de nascimento, sexo e grupos de manejo à desmama e ao sobreano) e idade do animal (covariável linear), e como aleatórios foram considerados os efeitos genético aditivo direto e residual. As estimativas dos coeficientes de herdabilidade foram 0,36 e 0,39 para peso aos 18 meses (PESSOB); 0,27 e 0,29 para ganho de peso da desmama aos 18 meses (GP345); 0,22 e 0,21 para...

Estimação de parâmetros genéticos para probabilidade de prenhez aos 14 meses e altura na garupa em bovinos da raça Nelore

Palavras-chave: Características de crescimento escore visual Método <; FONT FACE=Symbol>; Â<; /FONT>; Nelore parâmetros genéticos REML.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982003000700011

R. Bras. Zootec.

Silva,Josineudson Augusto II de Vasconcelos; Van Melis,Marcelo Hessel; Eler,Joanir Pereira; Ferraz,José Bento Sterman.

Registros de 24.703 animais da raça Nelore, provenientes de seis fazendas, foram utilizados para estimar os coeficientes de herdabilidade e a correlação genética relativos às características probabilidade de prenhez aos 14 meses (PP14) e altura na garupa aos 450 dias de idade (AG450). O modelo matemático incluiu os efeitos fixos de grupo de contemporâneo (181 grupos) e classe de idade da mãe ao parto (7 classes) para PP14 e o efeito fixo de grupo de contemporâneo (584 grupos) para AG450. Os efeitos aleatórios incluídos em ambos os modelos foram genético aditivo do touro e residual. Os componentes de variância e covariância foram obtidos pelo método Â. As estimativas de herdabilidade foram iguais a 0,73 ± 0,01 e 0,30 ±...

Gastroduodenal resistance and neural mechanisms involved in saline flow decrease elicited by acute blood volume expansion in anesthetized rats

Palavras-chave: Altura na garupa correlação genética herdabilidade método <; FONT FACE=Symbol>; Â<; /FONT>; Prenhez de novilha.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982003000500014

Graça,J.R.V.; Gondim,F. de-A.A.; Cavalcante,D.I.M.; Xavier-Neto,J.; Messias,E.L.M.; Rego,M.C.V.; Marques,J.A.P.; Santos,A.A.; Rola,F.H..

We have previously demonstrated that blood volume (BV) expansion decreases saline flow through the gastroduodenal (GD) segment in anesthetized rats (Xavier-Neto J, dos Santos AA & Rola FH (1990) Gut, 31: 1006-1010). The present study attempts to identify the site(s) of resistance and neural mechanisms involved in this phenomenon. Male Wistar rats (N = 97, 200-300 g) were surgically manipulated to create four gut circuits: GD, gastric, pyloric and duodenal. These circuits were perfused under barostatically controlled pressure (4 cmH2O). Steady-state changes in flow were taken to reflect modifications in circuit resistances during three periods of time: normovolemic control (20 min), expansion (10-15 min), and expanded (30 min). Perfusion flow rates...

IFN-g in human Chagas' disease: protection or pathology?

Palavras-chave: Blood volume expansion gastroduodenal resistance duodenum pylorus !-- $MVD$:face("Symbol") --<; FONT FACE=Symbol>; A<; /font>; -blockers vagotomy.

Ano: 1997

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000019

Bahia-Oliveira,L.M.G.; Gomes,J.A.S.; Rocha,M.O.C.; Moreira,M.C.V.; Lemos,E.M.; Luz,Z.M.P.; Pereira,M.E.S.; Coffman,R.L.; Dias,J.C.P.; Cançado,J.R.; Gazzinelli,G.; Corrêa-Oliveira,R..

An apparently paradoxical role for IFN-g in human Chagas' disease was observed when studying the pattern of cytokine production by peripheral blood mononuclear cells (PBMC) obtained from two groups of chagasic patients after specific stimulation with Trypanosoma cruzi-derived antigens. The groups studied were 1) patients treated with benznidazole during the acute phase of Trypanosoma cruzi infection and 2) chronically infected untreated patients. In the treated group, higher levels of IFN-g were produced by PBMC from individuals cured after treatment when compared to non-cured patients. In contrast, in the chronically infected group (not treated) higher levels of IFN-<FONT...

Immunologic patterns associated with cure in human American cutaneous leishmaniasis

Palavras-chave: Trypanosoma cruzi Chagas' disease cardiomyopathy peripheral blood mononuclear cells (PBMC) cytokines INF-<; FONT FACE=Symbol>; G<; /font>.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100017

Coutinho,S.G.; Da-Cruz,A.M.; Bertho,A.L.; Santiago,M.A.; De-Luca,P..

Patients with American cutaneous leishmaniasis were studied before therapy (active lesion) and at the end of therapy (cured patients). Assays of lymphocyte proliferative responses of peripheral blood mononuclear cells induced in vitro by Leishmania braziliensis promastigote antigens (Lb) were performed. Antigen-stimulated cells were harvested for CD4 and CD8 phenotype analysis and the levels of gamma interferon (IFN-g) and interleukin 4 (IL-4) produced were also determined in the culture supernatants. Two different patterns of Lb-induced T cell responses were observed: a) predominance of responding CD4+ cells and mixed type 1 and type 2 cytokine production (IFN-g and IL-4) during...

Insecticidal and antifungic proteins of the latex from Manihot glaziovii Muell. Arg.

Palavras-chave: Human cutaneous leishmaniasis Leishmania braziliensis CD4+ and CD8+ cells IFN-<; FONT FACE=Symbol>; G<; /font>; IL-4.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100019

Rev. Bras. Bot.

PEREIRA,LUCILENE S.; GOMES,VALDIRENE M.; FERNANDES,KÁTIA V. S.; SALES,MAURÍCIO P.; XAVIER-FILHO,JOSÉ.

Analysis of the latex from Manihot glaziovii showed the presence of various enzymatic and inhibitory activities. The latex also presented an inhibitory effect on the development of cowpea weevil (Callosobruchus maculatus) in an artificial seed system and on the development, in an in vitro assay, of phytopathogenic fungi Colletotrichum gloesporioides, Fusarium solani and Macrophomina phaseolina. These results suggest the presence of substances, some of them of protein nature, involved in plant defense mechanisms in this exudation product.

Interaction between NO and oxytocin: Influence on LHRH release

Palavras-chave: Latex chitinase <; FONT FACE=Symbol>; B<; /FONT>; -1 3-glucanase proteinase inhibitors defense proteins.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84041999000100005

Nitric oxide synthase (NOS)-containing neurons have been localized in various parts of the CNS. These neurons occur in the hypothalamus, mostly in the paraventricular and supraoptic nuclei and their axons project to the neural lobe of the pituitary gland. We have found that nitric oxide (NO) controls luteinizing hormone-releasing hormone (LHRH) release from the hypothalamus acting as a signal transducer in norepinephrine (NE)-induced LHRH release. LHRH not only releases LH from the pituitary but also induces sexual behavior. On the other hand, it is known that oxytocin also stimulates mating behavior and there is some evidence that oxytocin can increase NE release. Therefore, it occurred to us that oxytocin may also stimulate LHRH release via NE and NO. To...

INTERFERON SELECTIVELY INHIBITS THE SYNTHESIS OF MAYARO VIRUS GLYCOPROTEINS

Palavras-chave: Nitric oxide synthase norepinephrine <; FONT FACE=Symbol>; A<; /font>; -adrenergic receptors prostaglandin E2 arginine citrulline NMMA sodium nitroprusside.

Ano: 1997

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400004

Rev. Microbiol.

Ferreira,Davis F.; Rebello,Maria Christina Soares.

We have previously observed that interferon (recIFNa2b) blocks the process of morphogenesis of Mayaro virus in TC7 cells (monkey kidney). In this work we show that IFNa inhibits preferentially virus glycoproteins and their precursors, and this effect is probably correlated to the alterations in the morphogenesis process previously observed.

Multifactorial control of water and saline intake: role of a2-adrenoceptors

Palavras-chave: Interferon <; FONT FACE=Symbol>; A<; /FONT>; Mayaro virus glycoproteins morphogenesis.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300014

Water and saline intake is controlled by several mechanisms activated during dehydration. Some mechanisms, such as the production of angiotensin II and unloading of cardiovascular receptors, activate both behaviors, while others, such as the increase in blood osmolality or sodium concentration, activate water, but inhibit saline intake. Aldosterone probably activates only saline intake. Clonidine, an a2-adrenergic agonist, inhibits water and saline intake induced by these mechanisms. One model to describe the interactions between these multiple mechanisms is a wire-block diagram, where the brain circuit that controls each intake is represented by a summing point of its respective inhibiting and activating factors. The...